SMAD9 Polyklonaler Antikörper

SMAD9 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Rezensionen (1)

Kat-Nr. : 16397-1-AP

Synonyme

MAD homolog 9, MADH6, MADH9, Mothers against DPP homolog 9, SMAD 9

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• 

  WB analysis of HeLa using 16397-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16397-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16397-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 16397-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 16397-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16397-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16397-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16397-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HEK-293-Zellen, HepG2-Zellen, Mauslungengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

16397-1-AP bindet in WB, ELISA SMAD9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMAD9 fusion protein Ag9490
• Vollständiger Name: SMAD family member 9
• Berechnetes Molekulargewicht: 430 aa, 49 kDa
• Beobachtetes Molekulargewicht: 49-55 kDa
• GenBank-Zugangsnummer: BC011559
• Gene symbol: SMAD9
• Gene ID (NCBI): 4093
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMAD9 (also known as MADH9 or MAD homolog 9) is a key signaling molecule in the transforming growth factor-β (TGF-β) superfamily signaling pathway and belongs to the SMAD1/5/9 subgroup of receptor-regulated SMADs (R-SMADs). It is primarily activated and phosphorylated by BMP (bone morphogenetic protein) type I receptors. Once activated, SMAD9 forms a complex with the common SMAD4 (Co-SMAD), translocates into the nucleus, and acts as a transcription factor to regulate the expression of specific target genes. This process is involved in modulating a variety of critical biological processes, including cell proliferation, differentiation, apoptosis, and embryonic development. Mutations or abnormal expression of SMAD9 are associated with several diseases, particularly in pulmonary arterial hypertension, certain cancers, and vascular diseases, where it plays a significant role.

Publikationen

Species	Application	Title
human	WB	IUBMB Life; LncRNA RP11-301G19.1 is required for the maintenance of vascular smooth muscle cell contractile phenotype via sponging miR-17-5P/ATOH8 axis; Authors - Shuning Hao
mouse	WB	Sci Rep; Schisandrin B regulates MC3T3-E1 subclone 14 cells proliferation and differentiation through BMP2-SMADs-RUNX2-SP7 signaling axis.; Authors - Xueni Wang
mouse	WB	Cell Death Discov; Gsα deficiency facilitates cardiac remodeling via CREB/ Bmp10-mediated signaling; Authors - Ping Yin
	WB	Bioact Mater; A tantalum-containing zirconium-based metallic glass with superior endosseous implant relevant properties; Authors - Yunshu Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nadia (Verified Customer) (09-15-2025)

We used this SMAD9 antibody for both Western blot and immunofluorescence applications. The antibody produced a clear, specific band with minimal background.

• Applications: Western Blot, Immunohistochemistry