SMAD9 Polyklonaler Antikörper

SMAD9 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16397-1-PBS

Synonyme

MAD homolog 9, MADH6, MADH9, Mothers against DPP homolog 9, SMAD 9

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  WB analysis of HeLa using 16397-1-AP (same clone as 16397-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16397-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 16397-1-AP (same clone as 16397-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16397-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 16397-1-AP (same clone as 16397-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16397-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 16397-1-AP (same clone as 16397-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16397-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 16397-1-AP (same clone as 16397-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16397-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 16397-1-AP (same clone as 16397-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16397-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 16397-1-AP (same clone as 16397-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16397-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16397-1-AP (same clone as 16397-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16397-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16397-1-PBS bindet in WB, Indirect ELISA SMAD9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMAD9 fusion protein Ag9490
• Vollständiger Name: SMAD family member 9
• Berechnetes Molekulargewicht: 430 aa, 49 kDa
• Beobachtetes Molekulargewicht: 49-55 kDa
• GenBank-Zugangsnummer: BC011559
• Gene symbol: SMAD9
• Gene ID (NCBI): 4093
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMAD9 (also known as MADH9 or MAD homolog 9) is a key signaling molecule in the transforming growth factor-β (TGF-β) superfamily signaling pathway and belongs to the SMAD1/5/9 subgroup of receptor-regulated SMADs (R-SMADs). It is primarily activated and phosphorylated by BMP (bone morphogenetic protein) type I receptors. Once activated, SMAD9 forms a complex with the common SMAD4 (Co-SMAD), translocates into the nucleus, and acts as a transcription factor to regulate the expression of specific target genes. This process is involved in modulating a variety of critical biological processes, including cell proliferation, differentiation, apoptosis, and embryonic development. Mutations or abnormal expression of SMAD9 are associated with several diseases, particularly in pulmonary arterial hypertension, certain cancers, and vascular diseases, where it plays a significant role.