SMARCA2-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SMARCA2-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 26613-1-AP

Synonyme

SMARCA2, EC:3.6.4.-, BRM, BRG1-associated factor 190B, BAF190B

---

• 

  WB analysis of HeLa using 26613-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26613-1-AP (SMARCA2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 26613-1-AP

  WB result of SMARCA2-Specific antibody (26613-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMARCA2-Specific transfected HeLa cells.

• 

  WB analysis of NIH/3T3 using 26613-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26613-1-AP (SMARCA2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 26613-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 26613-1-AP (SMARCA2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 26613-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26613-1-AP (SMARCA2-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 26613-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26613-1-AP (SMARCA2-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 26613-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 26613-1-AP (SMARCA2-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 26613-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMARCA2-Specific antibody (26613-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, MDA-MB-453s-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• CoIP: See 1 publications below

Produktinformation

26613-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA SMARCA2-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMARCA2-Specific fusion protein Ag25157
• Vollständiger Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
• Berechnetes Molekulargewicht: 181 kDa
• Beobachtetes Molekulargewicht: 180-190 kDa
• GenBank-Zugangsnummer: NM_003070
• Gene symbol: SMARCA2
• Gene ID (NCBI): 6595
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMARCA2 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. SMARCA2 is involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Publikationen

Species	Application	Title
rat	WB	Neuron; Nucleoporin Seh1 Interacts with Olig2/Brd7 to Promote Oligodendrocyte Differentiation and Myelination.; Authors - Zhixiong Liu
human	WB	Oncol Lett; PMID: 30127991; Authors - Xiaoli Xu
mouse	WB	Stem Cells; Chromatin Remodeling Factor BRG1 Regulates Stemness and Chemosensitivity of Glioma Initiating Cells.; Authors - Debolina Ganguly
mouse	WB	Biochem Biophys Res Commun; Chromatin remodeler BRM is a key mediator of leucine-stimulated mTOR gene transcription in mouse mammary epithelial cells; Authors - Changping Ke
human	CoIP	Nat Commun; Prion-like domain mediated phase separation of ARID1A promotes oncogenic potential of Ewing's sarcoma; Authors - Yong Ryoul Kim
human	WB	Cell Rep; SWI/SNF complex-mediated ZNF410 cooperative binding maintains chromatin accessibility and enhancer activity; Authors - Siyuan Xu