SMARCA4/BRG1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

SMARCA4/BRG1 Monoklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

2E6B6

Kat-Nr. : 66561-1-Ig

Synonyme

SMARCA4, 2E6B6, ATP dependent helicase SMARCA4, ATP-dependent helicase SMARCA4, BAF190

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  WB analysis of HepG2 using 66561-1-Ig

  WB result of SMARCA4/BRG1 antibody (66561-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMARCA4/BRG1 transfected HepG2 cells.

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  WB analysis using 66561-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66561-1-Ig (SMARCA4/BRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 66561-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66561-1-Ig (SMARCA4/BRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 66561-1-Ig

  IP result of anti-SMARCA4/BRG1 (IP:66561-1-Ig, 5ug; Detection:66561-1-Ig 1:3000) with HeLa cells lysate 3200 ug.

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  IF Staining of HepG2 using 66561-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMARCA4/BRG1 antibody (66561-1-Ig, Clone: 2E6B6 ) at dilution of 1:50000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of PC-3 using 66561-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using SMARCA4/BRG1 antibody (66561-1-Ig, Clone: 2E6B6 ) at dilution of 1:50000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 66561-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMARCA4/BRG1 antibody (66561-1-Ig, Clone: 2E6B6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, 4T1-Zellen, COLO 320-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

66561-1-Ig bindet in WB, IF/ICC, IP, ChIP, ELISA SMARCA4/BRG1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SMARCA4/BRG1 fusion protein Ag16256
• Vollständiger Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
• Berechnetes Molekulargewicht: 1647 aa, 185 kDa
• Beobachtetes Molekulargewicht: 185 kDa
• GenBank-Zugangsnummer: BC150298
• Gene symbol: SMARCA4
• Gene ID (NCBI): 6597
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Publikationen

Species	Application	Title
human	WB,IF	Inflammation; Regulation of KDM2B and Brg1 on Inflammatory Response of Nasal Mucosa in CRSwNP.; Authors - C C Liu ; KD Validated
mouse	ChIP	Front Pharmacol; Brg1 and RUNX1 synergy in regulating TRPM4 channel in mouse cardiomyocytes; Authors - Tao Ban