SMARCA4/BRG1 Rekombinanter Antikörper

SMARCA4/BRG1 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240172F4

Kat-Nr. : 83310-2-RR

Synonyme

SMARCA4, 240172F4, ATP dependent helicase SMARCA4, ATP-dependent helicase SMARCA4, BAF190

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  WB analysis using 83310-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83310-2-RR (SMARCA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 83310-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with .

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  IHC staining of mouse colon using 83310-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with .

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  IHC staining of human placenta using 83310-2-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 83310-2-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 83310-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 83310-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 83310-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 83310-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 83310-2-RR

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 83310-2-RR

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 83310-2-RR

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 83310-2-RR

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 83310-2-RR

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 83310-2-RR

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 83310-2-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 83310-2-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83310-2-RR (SMARCA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 83310-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMARCA4 antibody (83310-2-RR, Clone: 240172F4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of Jurkat using 83310-2-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug SMARCA4 Recombinant antibody (83310-2-RR, Clone:240172F4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83310-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83310-2-RR against Human SMARCA4 were performed. The affinity constant is 3.66 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, HEK-293T-Zellen, HepG2-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Plazenta-Gewebe, Maus-Cerebellum-Gewebe, Maus-Kolongewebe, Mauslungengewebe, Ratten-Cerebellum-Gewebe, Ratten-Kolongewebe, Rattenlungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83310-2-RR bindet in WB, IHC, IF/ICC, FC (Intra), ELISA SMARCA4/BRG1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SMARCA4/BRG1 fusion protein Ag16256
• Vollständiger Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
• Berechnetes Molekulargewicht: 1647 aa, 185 kDa
• Beobachtetes Molekulargewicht: 185 kDa
• GenBank-Zugangsnummer: BC150298
• Gene symbol: SMARCA4
• Gene ID (NCBI): 6597
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.