SMARCA4 Monoklonaler Antikörper

SMARCA4 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

2E6B6

Kat-Nr. : CL594-66561

Synonyme

ATP dependent helicase SMARCA4, BAF190, BAF190A, BRG1, BRG1 associated factor 190A, FLJ39786, hSNF2b, Protein brahma homolog 1, Protein BRG 1, SMARCA4, SNF2, SNF2 BETA, SNF2B, SNF2L4, SNF2LB, SWI2, Transcription activator BRG1

---

Galerie der Validierungsdaten

• 

  IF Staining of HepG2 using CL594-66561

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 SMARCA4 antibody (CL594-66561, Clone: 2E6B6 ) at dilution of 1:200. DAPI (blue).

• 

  IF Staining of HepG2 using CL594-66561

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL594-66561 (SMARCA4 antibody) at dilution of 1:50.

• 

  FC experiment of HeLa using CL594-66561

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human SMARCA4 (CL594-66561, Clone:2E6B6) (red), or 0.2 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66561 bindet in IF, FC (Intra) SMARCA4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SMARCA4 fusion protein Ag16256
• Vollständiger Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
• Berechnetes Molekulargewicht: 1647 aa, 185 kDa
• GenBank-Zugangsnummer: BC150298
• Gene symbol: SMARCA4
• Gene ID (NCBI): 6597
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. And this anitbody is CL594(Ex/Em 593 nm/614 nm) conjugated.

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011