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SMARCA4/BRG1 Polyklonaler Antikörper

SMARCA4/BRG1 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20650-1-PBS

Synonyme

SMARCA4, BRG1 associated factor 190A, BRG1, BAF190A, BAF190

Filter:
  • Western Blot (WB) analysis of HeLa cells using SMARCA4/BRG1 Polyclonal antibody (20650-1-AP)

    WB analysis of HeLa using 20650-1-AP (same clone as 20650-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20650-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20650-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SMARCA4/BRG1 Polyclonal antibody (20650-1-AP)

    WB analysis of HepG2 using 20650-1-AP (same clone as 20650-1-PBS)

    WB result of SMARCA4/BRG1 antibody (20650-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMARCA4/BRG1 transfected HepG2 cells. This data was developed using the same antibody clone with 20650-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using SMARCA4/BRG1 Polyclonal antibody (20650-1-AP)

    WB analysis of K-562 using 20650-1-AP (same clone as 20650-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 20650-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20650-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

20650-1-PBS bindet in WB, Indirect ELISA SMARCA4/BRG1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Berechnetes Molekulargewicht 185 kDa
Beobachtetes Molekulargewicht 180 kDa
GenBank-ZugangsnummerNM_001128844
Gene symbol SMARCA4
Gene ID (NCBI) 6597
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. The antibody is specific to SMARCA4.