SMARCA4/BRG1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

SMARCA4/BRG1 Monoklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

2E6B6

Kat-Nr. : 66561-1-PBS

Synonyme

SMARCA4, 2E6B6, ATP dependent helicase SMARCA4, ATP-dependent helicase SMARCA4, BAF190

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  WB analysis of HepG2 using 66561-1-Ig (same clone as 66561-1-PBS)

  WB result of SMARCA4/BRG1 antibody (66561-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMARCA4/BRG1 transfected HepG2 cells. This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

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  WB analysis using 66561-1-Ig (same clone as 66561-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66561-1-Ig (SMARCA4/BRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 66561-1-Ig (same clone as 66561-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66561-1-Ig (SMARCA4/BRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 66561-1-Ig (same clone as 66561-1-PBS)

  IP result of anti-SMARCA4/BRG1 (IP:66561-1-Ig, 5ug; Detection:66561-1-Ig 1:3000) with HeLa cells lysate 3200 ug. This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66561-1-Ig (same clone as 66561-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMARCA4/BRG1 antibody (66561-1-Ig, Clone: 2E6B6 ) at dilution of 1:50000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 66561-1-Ig (same clone as 66561-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using SMARCA4/BRG1 antibody (66561-1-Ig, Clone: 2E6B6 ) at dilution of 1:50000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66561-1-Ig (same clone as 66561-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMARCA4/BRG1 antibody (66561-1-Ig, Clone: 2E6B6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66561-1-PBS bindet in WB, IF/ICC, IP, ELISA SMARCA4/BRG1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SMARCA4/BRG1 fusion protein Ag16256
• Vollständiger Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
• Berechnetes Molekulargewicht: 1647 aa, 185 kDa
• Beobachtetes Molekulargewicht: 185 kDa
• GenBank-Zugangsnummer: BC150298
• Gene symbol: SMARCA4
• Gene ID (NCBI): 6597
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.