SMARCC1 Rekombinanter Antikörper

SMARCC1 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250455A1

Kat-Nr. : 86015-1-PBS

Synonyme

BAF155, BRG1-associated factor 155, CRACC1, Rsc8, SRG3

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  WB analysis using 86015-1-RR (same clone as 86015-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86015-1-RR (SMARCC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86015-1-PBS in a different storage buffer formulation.

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  Sample test of MP02222-1

  The mean SMARCC1 concentration was determined to be 130.2 ng/mL in Jurkat cell extract based on a 2.2 mg/mL extract load.

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  IF Staining of Jurkat using 86015-1-RR (same clone as 86015-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SMARCC1 antibody (86015-1-RR, Clone: 250455A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86015-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP02222-1

  Cytometric bead array standard curve of MP02222-1, SMARCC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86015-1-PBS. Detection antibody: 86015-2-PBS. Standard: Ag12109. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 86015-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86015-1-RR against Human SMARCC1 were performed. The affinity constant is 0.393 nM.

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Geprüfte Anwendungen

Produktinformation

86015-1-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test SMARCC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SMARCC1 fusion protein Ag12109
• Vollständiger Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1
• Berechnetes Molekulargewicht: 1105 aa, 123 kDa
• Beobachtetes Molekulargewicht: 155-160 kDa
• GenBank-Zugangsnummer: BC050564
• Gene symbol: SMARCC1
• Gene ID (NCBI): 6599
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMARCC1, also named as SWI/SNF complex 155 kDa subunit or BAF155, is a 1105 amino acid protein, which belongs to the SMARCC family. SMARCC1 is expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas. SMARCC1 is Involved in transcriptional activation and repression of select genes by chromatin remodeling. SMARCC1 may stimulate the ATPase activity of the catalytic subunit of the complex. SMARCC1 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). The calculated molecular weight of TCEA1 is 123 kDa, but modified SMARCC1 is about 155-160 kDa. (PMID: 24025402)