SMARCD1 Polyklonaler Antikörper

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• KD/KO Validated

SMARCD1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10998-2-PBS

Synonyme

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  WB analysis of mouse brain using 10998-2-AP (same clone as 10998-2-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10998-2-AP (SMARCD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10998-2-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 10998-2-AP (same clone as 10998-2-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10998-2-AP (SMARCD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10998-2-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 10998-2-AP (same clone as 10998-2-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10998-2-AP (SMARCD1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10998-2-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 10998-2-AP (same clone as 10998-2-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 10998-2-AP (SMARCD1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10998-2-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 10998-2-AP (same clone as 10998-2-PBS)

  IP result of anti-SMARCD1 (IP:10998-2-AP, 3ug; Detection:10998-2-AP 1:600) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 10998-2-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 10998-2-AP (same clone as 10998-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10998-2-AP (SMARCD1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10998-2-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 10998-2-AP (same clone as 10998-2-PBS)

  Immunofluorescent analysis of SH-SY5Y cells, using SMARCD1 antibody 10998-2-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 10998-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10998-2-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA SMARCD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMARCD1 fusion protein Ag1453
• Vollständiger Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC009368
• Gene symbol: SMARCD1
• Gene ID (NCBI): 6602
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMARCD1 belongs to the nuclear SWI/SNF chromatin remodeling complex, which regulates transcription and interacts with nucleolin in the nucleus. In addition, it's a member of the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). It possesses a SWIB domain which is homologous to and shares a common fold with the p53-binding domain of MDM2, a cell cycle regulator which interacts with NPM1. SMARCD1 has a strong influence on the Vitamin D-mediated transcriptional activity from an enhancer Vitamin D receptor element (VDRE). And it can mediates critical interactions between nuclear receptors and the BRG1/SMARCA4 chromatin-remodeling complex for transactivation. This antibody may have across reaction with SMARCD2.