SMARCD2 Polyklonaler Antikörper

SMARCD2 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11156-1-PBS

Synonyme

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  WB analysis of RAW264.7 using 11156-1-AP (same clone as 11156-1-PBS)

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11156-1-AP (SMARCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11156-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 11156-1-AP (same clone as 11156-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11156-1-AP (SMARCD2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 11156-1-AP (same clone as 11156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11156-1-AP (SMARCD2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 11156-1-AP (same clone as 11156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11156-1-AP (SMARCD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 11156-1-AP (same clone as 11156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11156-1-AP (SMARCD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 11156-1-AP (same clone as 11156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 11156-1-AP (SMARCD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 11156-1-AP (same clone as 11156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 11156-1-AP (SMARCD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11156-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11156-1-PBS bindet in WB, IHC, Indirect ELISA SMARCD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMARCD2 fusion protein Ag1630
• Vollständiger Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC018953
• Gene symbol: SMARCD2
• Gene ID (NCBI): 6603
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The SWI/SNF chromatin remodelling complexes are important regulators of transcription; they consist of large multisubunit assemblies containing either Brm or Brg1 as the catalytic ATPase subunit and a variable subset of approximately 10 Brg/Brm-associated factors (BAF) [PMID:20148946]. SMARCD2 is one of BAF60 proteins, which are found in most complexes, is thought to bridge interactions between transcription factors and SWI/SNF complexes. [PMID:9427560]