SMC3 Polyklonaler Antikörper

SMC3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14185-1-PBS

Synonyme

Chromosome-associated polypeptide, CDLS3, BMH, Basement membrane-associated chondroitin proteoglycan, Bamacan

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  WB analysis of HeLa using 14185-1-AP (same clone as 14185-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14185-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 14185-1-AP (same clone as 14185-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14185-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 14185-1-AP (same clone as 14185-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 14185-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 14185-1-AP (same clone as 14185-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14185-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 14185-1-PBS

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IHC staining of human stomach using 14185-1-AP (same clone as 14185-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14185-1-AP (SMC3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14185-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 14185-1-AP (same clone as 14185-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14185-1-AP (SMC3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14185-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14185-1-PBS bindet in WB, IHC, IF/ICC, ELISA SMC3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMC3 fusion protein Ag5440
• Vollständiger Name: structural maintenance of chromosomes 3
• Berechnetes Molekulargewicht: 142 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC047324
• Gene symbol: SMC3
• Gene ID (NCBI): 9126
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.