SMC4 Polyklonaler Antikörper

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• KD/KO Validated

SMC4 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24758-1-PBS

Synonyme

CAP C, CAPC, Chromosome-associated polypeptide C, hCAP C, hCAPC

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  WB analysis using 24758-1-AP (same clone as 24758-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24758-1-AP (SMC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 24758-1-AP (same clone as 24758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 24758-1-AP (same clone as 24758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 24758-1-AP (same clone as 24758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 24758-1-AP (SMC4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 24758-1-AP (same clone as 24758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 24758-1-AP (same clone as 24758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 24758-1-AP (same clone as 24758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 24758-1-AP (same clone as 24758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 24758-1-AP (same clone as 24758-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMC4 antibody (24758-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

24758-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SMC4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMC4 fusion protein Ag19156
• Vollständiger Name: structural maintenance of chromosomes 4
• Berechnetes Molekulargewicht: 1288 aa, 147 kDa
• Beobachtetes Molekulargewicht: 147-150 kDa
• GenBank-Zugangsnummer: BC106033
• Gene symbol: SMC4
• Gene ID (NCBI): 10051
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMC4, also named as Structural maintenance of chromosomes protein 4, is a 1288 amino acid protein, which is widely expressed and belongs to the SMC family, SMC4 subfamily. SMC4 is a Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The calculated molecular weight of SMC4 is 147 kDa, but modified SMC4 is about 160 kDa.