SMC6 Polyklonaler Antikörper

SMC6 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14465-1-PBS

Synonyme

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  WB analysis using 14465-1-AP (same clone as 14465-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14465-1-AP (SMC6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14465-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 14465-1-AP (same clone as 14465-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14465-1-AP (SMC6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14465-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 14465-1-AP (same clone as 14465-1-PBS)

  IP result of anti-SMC6 (IP:14465-1-AP, 4ug; Detection:14465-1-AP 1:1000) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 14465-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 14465-1-AP (same clone as 14465-1-PBS)

  IP result of anti-SMC6 (IP:14465-1-AP, 4ug; Detection:14465-1-AP 1:4000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 14465-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 14465-1-AP (same clone as 14465-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14465-1-AP (SMC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14465-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14465-1-PBS bindet in WB, IHC, IP, Indirect ELISA SMC6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMC6 fusion protein Ag5812
• Vollständiger Name: structural maintenance of chromosomes 6
• Berechnetes Molekulargewicht: 126 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC039828
• Gene symbol: SMC6
• Gene ID (NCBI): 79677
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMC6 (Structural maintenance of chromosomes protein 6) is core component of the SMC5-SMC6 complex that is involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs) (PMID: 26983541).