SMCR7/MID49 Polyklonaler Antikörper

SMCR7/MID49 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 28718-1-AP

Synonyme

MID49, SMCR7

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  WB analysis using 28718-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28718-1-AP (SMCR7,MID49 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 28718-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28718-1-AP (SMCR7,MID49 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28718-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28718-1-AP (SMCR7,MID49 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below

Produktinformation

28718-1-AP bindet in WB, IHC, ELISA SMCR7/MID49 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMCR7/MID49 fusion protein Ag30119
• Vollständiger Name: Smith-Magenis syndrome chromosome region, candidate 7
• Berechnetes Molekulargewicht: 454 aa, 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC014973
• Gene symbol: MID49
• Gene ID (NCBI): 125170
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMCR7, also named as MID49, is a 49 kDa mitochondrial outer membrane protein which regulates mitochondrial morphology. SMCR7 can directly recruit the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. SMCR7 has two isoforms.

Publikationen

Species	Application	Title
pig	WB	Mol Med; Characterisation of cold-induced mitochondrial fission in porcine aortic endothelial cells.; Authors - Leonard Quiring
mouse	WB	Cell Rep; A PKD-MFF signaling axis couples mitochondrial fission to mitotic progression.; Authors - Evanthia Pangou
human	WB	Nat Commun; Auranofin targets UBA1 and enhances UBA1 activity by facilitating ubiquitin trans-thioesterification to E2 ubiquitin-conjugating enzymes; Authors - Wenjing Yan
mouse	WB	Int J Biol Sci; Mitochondrial Dysfunction by FADDosome Promotes Gastric Mucosal Injury in Portal Hypertensive Gastropathy; Authors - Yuelin Xiao
human	WB	Technol Cancer Res Treat; The Role of MIEF2 in Cisplatin Sensitivity in KIRP Patients: Insights from Four-gene Mitochondrial Fusion RNA Markers; Authors - Yusong Hou
monkey	WB	PLoS Pathog; Calcium-mediated mitochondrial fission and mitophagy drive glycolysis to facilitate arterivirus proliferation; Authors - Zhe Sun ; KD Validated