SMCR7L Monoklonaler Antikörper

SMCR7L Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3B3G3

Kat-Nr. : 67808-1-PBS

Synonyme

dJ1104E15.3, FLJ20232, HSU79252, MID51, MIEF1, SMCR7L

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  WB analysis using 67808-1-Ig (same clone as 67808-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67808-1-Ig (SMCR7L/MID51 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67808-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 67808-1-Ig (same clone as 67808-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67808-1-Ig (SMCR7L/MID51 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67808-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 67808-1-Ig (same clone as 67808-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67808-1-Ig (SMCR7L/MID51 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67808-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 67808-1-Ig (same clone as 67808-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SMCR7L/MID51 antibody (67808-1-Ig, Clone: 3B3G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67808-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67808-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA SMCR7L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SMCR7L fusion protein Ag13775
• Vollständiger Name: Smith-Magenis syndrome chromosome region, candidate 7-like
• Berechnetes Molekulargewicht: 463 aa, 51 kDa
• Beobachtetes Molekulargewicht: 48-51 kDa
• GenBank-Zugangsnummer: BC002587
• Gene symbol: SMCR7L
• Gene ID (NCBI): 54471
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human SMCR7L gene encodes, MID51, the mitochondrial dynamic protein of 51 kDa (also called mitochondrial elongation factor 1, MIEF1). MID51 is a single-pass membrane protein anchored to the mitochondrial outer membrane and regulates mitochondrial morphology. Mitochondrial morphology is controlled by two opposing processes: fusion and fission. Elevated MID51 levels induce extensive mitochondrial fusion, whereas depletion of MID51 causes mitochondrial fragmentation. MID51 interacts with and recruits Drp1 to mitochondria, suggesting a critical role of MID51 in regulation of mitochondrial fusion-fission machinery in vertebrates.