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SMEK1 Polyklonaler Antikörper

SMEK1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26153-1-PBS

Synonyme

KIAA2010, PP4R3A, PPP4R3A

Filter:
  • Western Blot (WB) analysis of various lysates using SMEK1 Polyclonal antibody (26153-1-AP)

    WB analysis using 26153-1-AP (same clone as 26153-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26153-1-AP (SMEK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26153-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SMEK1 Polyclonal antibody (26153-1-AP)

    IHC staining of human colon cancer using 26153-1-AP (same clone as 26153-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26153-1-AP (SMEK1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26153-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SMEK1 Polyclonal antibody (26153-1-AP)

    IF Staining of MCF-7 using 26153-1-AP (same clone as 26153-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SMEK1 antibody (26153-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 26153-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

26153-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SMEK1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SMEK1 fusion protein Ag5070
Vollständiger Name SMEK homolog 1, suppressor of mek1 (Dictyostelium)
Berechnetes Molekulargewicht 833 aa, 95 kDa
Beobachtetes Molekulargewicht95-100 kDa
GenBank-ZugangsnummerBC038932
Gene symbol SMEK1
Gene ID (NCBI) 55671
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPP4R3A also known as SMEK1, is the regulatory subunit of serine/threonine-protein phosphatase 4. SMEK1 regulates the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on 'Ser-140' (gamma-H2AX) generated during DNA replication and required for DNA DSB repair(PMID: 18614045).