SMMHC Monoklonaler Antikörper

SMMHC Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

5C6D3

Kat-Nr. : 60222-1-PBS

Synonyme

MYH11, 5C6D3, KIAA0866, Myosin 11, Myosin heavy chain 11

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  WB analysis using 60222-1-Ig (same clone as 60222-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60222-1-Ig (same clone as 60222-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60222-1-Ig (same clone as 60222-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

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  WB analysis of human ileum using 60222-1-Ig (same clone as 60222-1-PBS)

  human ileum tissue were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast hyperplasia using 60222-1-Ig (same clone as 60222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast hyperplasia using 60222-1-Ig (same clone as 60222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60222-1-Ig (same clone as 60222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast hyperplasia using 60222-1-Ig (same clone as 60222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60222-1-PBS bindet in WB, IHC, ELISA SMMHC und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SMMHC fusion protein Ag13397
• Vollständiger Name: myosin, heavy chain 11, smooth muscle
• Berechnetes Molekulargewicht: 1938 aa, 224 kDa
• Beobachtetes Molekulargewicht: 220 kDa
• GenBank-Zugangsnummer: BC101677
• Gene symbol: SMMHC/MYH11
• Gene ID (NCBI): 4629
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMMHC (smooth muscle myosin heavy chain; MYH11) is a contractile protein of smooth muscle cells. It is specifically expressed in cells derived from smooth muscle lineages. SMMHC is used as vascular smooth muscle cell (vSMC) contractile marker. It is also an excellent marker for myoepithelial cells, with no or few cross-reaction with myofibroblasts, thus very useful in the evaluation of stromal invasion.