SMN Polyklonaler Antikörper

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• KD/KO Validated

SMN Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11708-1-PBS

Synonyme

C BCD541, Component of gems 1, Gemin 1, Gemin-1, SMN1

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  WB analysis of HeLa using 11708-1-AP (same clone as 11708-1-PBS)

  WB result of SMN antibody (11708-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMN transfected HeLa cells. This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  WB analysis using 11708-1-AP (same clone as 11708-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11708-1-AP (SMN antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 11708-1-PBS

  K-562 cells were subjected to SDS PAGE followed by western blot with 11708-1-AP (SMN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11708-1-AP (same clone as 11708-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11708-1-AP (SMN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 11708-1-AP (same clone as 11708-1-PBS)

  IP result of anti-SMN (IP:11708-1-AP, 4ug; Detection:11708-1-AP 1:2000) with HEK-293 cells lysate 1040 ug. This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11708-1-AP (SMN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11708-1-AP (SMN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11708-1-AP (SMN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11708-1-AP (SMN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11708-1-AP (SMN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11708-1-AP (SMN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 11708-1-AP (SMN antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 11708-1-AP (same clone as 11708-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN antibody (11708-1-AP) at dilution of 1:1500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11708-1-PBS bindet in WB, IHC, IF/ICC, IP, ELISA SMN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMN fusion protein Ag2260
• Vollständiger Name: survival of motor neuron 2, centromeric
• Berechnetes Molekulargewicht: 282 aa, 30 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC000908
• Gene symbol: SMN
• Gene ID (NCBI): 6607
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA. This antibody, 11708-1-AP, raised against the recombinant full-length human SMN2 protein, recognizes all isoforms of SMN protein.