SMN-Exon7 Monoklonaler Antikörper

SMN-Exon7 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3A8G11

Kat-Nr. : 60255-1-PBS

Synonyme

SMN, 3A8G11, Component of gems 1, Gemin 1, Gemin-1

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  WB analysis of HEK-293 using 60255-1-Ig (same clone as 60255-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60255-1-Ig (same clone as 60255-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60255-1-Ig (SMN-Exon7 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60255-1-Ig (same clone as 60255-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60255-1-Ig (SMN-Exon7 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 60255-1-Ig (same clone as 60255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 60255-1-Ig (same clone as 60255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 60255-1-Ig (same clone as 60255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 60255-1-Ig (same clone as 60255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 60255-1-Ig (same clone as 60255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 60255-1-Ig (same clone as 60255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 60255-1-Ig (same clone as 60255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 60255-1-Ig (same clone as 60255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat pancreas using 60255-1-Ig (same clone as 60255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 60255-1-Ig (same clone as 60255-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SMN-Exon7 antibody (60255-1-Ig, Clone: 3A8G11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60255-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SMN-Exon7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SMN-Exon7 fusion protein Ag16615
• Vollständiger Name: survival of motor neuron 1, telomeric
• Berechnetes Molekulargewicht: 294 aa, 32 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC062723
• Gene symbol: SMN
• Gene ID (NCBI): 6606
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA. This antibody, 60255-1-Ig, raised against the C-terminal region (275-294aa) encoded by the exon 7.