SMN-Exon7 Monoklonaler Antikörper

SMN-Exon7 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

3A8G11

Kat-Nr. : 60255-1-Ig

Synonyme

SMN, 3A8G11, Component of gems 1, Gemin 1, Gemin-1

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  WB analysis of HEK-293 using 60255-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 60255-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60255-1-Ig (SMN-Exon7 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 60255-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60255-1-Ig (SMN-Exon7 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 60255-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 60255-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 60255-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 60255-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 60255-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 60255-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 60255-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 60255-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 60255-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 60255-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SMN-Exon7 antibody (60255-1-Ig, Clone: 3A8G11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: Mausherzgewebe, Maushirngewebe, Maus-Pankreasgewebe, Rattenhirngewebe, Rattenherzgewebe, Ratten-Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

60255-1-Ig bindet in WB, IHC, IF/ICC, ELISA SMN-Exon7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SMN-Exon7 fusion protein Ag16615
• Vollständiger Name: survival of motor neuron 1, telomeric
• Berechnetes Molekulargewicht: 294 aa, 32 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC062723
• Gene symbol: SMN
• Gene ID (NCBI): 6606
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA. This antibody, 60255-1-Ig, raised against the C-terminal region (275-294aa) encoded by the exon 7.

Publikationen

Species	Application	Title
Mouse	WB	Stem Cell Res; MicroRNA-29a promotes smooth muscle cell differentiation from stem cells by targeting YY1.; Authors - Min Jin
	WB	Science; Base editing rescue of spinal muscular atrophy in cells and in mice; Authors - Mandana Arbab