SMN Polyklonaler Antikörper

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• KD/KO Validated

SMN Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 22329-1-AP

Synonyme

C BCD541, Component of gems 1, Gemin 1, Gemin-1, SMN1

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  WB analysis of HEK-293 using 22329-1-AP

  WB result of SMN antibody (22329-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMN transfected HEK-293 cells.

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  WB analysis of K-562 using 22329-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 22329-1-AP (SMN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 22329-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22329-1-AP (SMN antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22329-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22329-1-AP (SMN antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 22329-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22329-1-AP (SMN antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 22329-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22329-1-AP (SMN antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 22329-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22329-1-AP (SMN antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 22329-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN antibody (22329-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

22329-1-AP bindet in WB, IHC, IF/ICC, ELISA SMN und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMN fusion protein Ag17798
• Vollständiger Name: survival of motor neuron 2, centromeric
• Berechnetes Molekulargewicht: 282 aa, 30 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC000908
• Gene symbol: SMN
• Gene ID (NCBI): 6607
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA.

Publikationen

Species	Application	Title
human	WB,IF	J Cell Sci; Dissecting the role of SMN multimerization in its dissociation from the Cajal body using harmine as a tool compound; Authors - Saki Ohazama