SMN (Human-Specific) Monoklonaler Antikörper

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• KD/KO Validated

SMN (Human-Specific) Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

2C6D9

Kat-Nr. : 60154-1-Ig

Synonyme

SMN, 2C6D9, C BCD541, Component of gems 1, Gemin 1

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  WB analysis of A375 using 60154-1-Ig

  A375, HEK-293, and Jurkat cells were subjected to SDS PAGE followed by western blot with 60154-1-Ig (SMN (Human-Specific) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 60154-1-Ig

  WB result of SMN (Human-Specific) antibody (60154-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMN (Human-Specific) transfected HEK-293 cells.

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  WB analysis of Raji using 60154-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 60154-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 60154-1-Ig

  IP result of anti-SMN (Human-Specific) (IP:60154-1-Ig, 4ug; Detection:60154-1-Ig 1:500) with HEK-293 cells lysate 2440ug.

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  IHC staining of human colon cancer using 60154-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 60154-1-Ig (SMN (Human-Specific) antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 60154-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 60154-1-Ig (SMN (Human-Specific) antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 60154-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60154-1-Ig (SMN (Human-Specific) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 60154-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60154-1-Ig (SMN (Human-Specific) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 60154-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 60154-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 60154-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 60154-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 60154-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 60154-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 60154-1-Ig(SMN (Human-Specific) antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 60154-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN (Human-Specific) antibody (60154-1-Ig, Clone: 2C6D9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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  FC experiment of Jurkat using 60154-1-Ig

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug SMN (Human-Specific) Monoclonal antibody (60154-1-Ig, Clone: 2C6D9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, Raji-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Herzgewebe, humanes Nierengewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

60154-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA SMN (Human-Specific) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SMN (Human-Specific) fusion protein Ag14333
• Vollständiger Name: survival of motor neuron 2, centromeric
• Berechnetes Molekulargewicht: 282 aa, 30 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC000908
• Gene symbol: SMN
• Gene ID (NCBI): 6607
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The survival of motor neurons (SMN) genes are the disease genes of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The level of SMN protein correlates with phenotypic severity of SMA. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional, because a large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein. This antibody 60154-1-Ig is specific to human SMN2. It can't recognize mouse and rat SMN.

Publikationen

Species	Application	Title
human		Nat Chem Biol; SMN2 splice modulators enhance U1-pre-mRNA association and rescue SMA mice.; Authors - James Palacino
human	WB	Science; Base editing rescue of spinal muscular atrophy in cells and in mice; Authors - Mandana Arbab