SMN Rekombinanter Antikörper

SMN Rekombinant Antikörper für WB, IF/ICC, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251620C4

Kat-Nr. : 86668-2-PBS

Synonyme

C BCD541, Component of gems 1, Gemin 1, Gemin-1, SMN1

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  WB analysis using 86668-2-RR (same clone as 86668-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86668-2-RR (SMN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86668-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02580-1

  The mean SMN concentration was determined to be 13.66 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02580-1

  Sandwich ELISA standard curve of MP02580-1, Human SMN Recombinant Matched Antibody Pair - PBS only. 86668-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg4812. 86668-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

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  IP experiment of HEK-293 using 86668-2-RR (same clone as 86668-2-PBS)

  IP result of anti-SMN (IP: 86668-2-RR, 4 ug; Detection: 86668-2-RR 1:600) with HEK-293 cells lysate 1200 ug. This data was developed using the same antibody clone with 86668-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86668-2-RR (same clone as 86668-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN antibody (86668-2-RR, Clone: 251620C4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86668-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86668-2-RR (same clone as 86668-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMN antibody (86668-2-RR, Clone: 251620C4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86668-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array sample test of MP02580-1

  The mean SMN concentration was determined to be 4.5 ng/mL in HeLa cell extract based on a 1.1 mg/mL extract load.

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  Cytometric bead array standard curve of MP02580-1

  Cytometric bead array standard curve of MP02580-1, SMN Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86668-2-PBS. Detection antibody: 86668-1-PBS. Standard: Eg4812. Range: 0.312-40 ng/mL.

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Geprüfte Anwendungen

Produktinformation

86668-2-PBS bindet in WB, IF/ICC, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA SMN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SMN fusion protein Eg4812
• Vollständiger Name: survival of motor neuron 2, centromeric
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC015308
• Gene symbol: SMN
• Gene ID (NCBI): 6607
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA.