SMPD1,ASM Polyklonaler Antikörper

SMPD1,ASM Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30197-1-AP

Synonyme

SMPD1, Sphingomyelin phosphodiesterase, EC:3.1.4.12, aSMase, ASM

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  WB analysis of human placenta using 30197-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30197-1-AP (SMPD1,ASM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 30197-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using SMPD1,ASM antibody (30197-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30197-1-AP bindet in WB, IF/ICC, ELISA SMPD1,ASM und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMPD1,ASM fusion protein Ag32479
• Vollständiger Name: sphingomyelin phosphodiesterase 1, acid lysosomal
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 68-70 kDa
• GenBank-Zugangsnummer: BC041164
• Gene symbol: SMPD1
• Gene ID (NCBI): 6609
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMPD1(Sphingomyelin phosphodiesterase) is also named as ASM and belongs to the acid sphingomyelinase family. It is an important enzyme in sphingolipid metabolism and plays key roles in apoptosis, immunity, development, and cancer. The deficient activity of this enzyme results in Types A and B Niemann-Pick disease (NPD). This protein has 4 isoforms produced by alternative splicing. SMPD1 can produce a 60 kDa peptide with its signal peptide missing. As a catalytically inactive 75kDa precursor, after signal peptide cleavage, it was processed into smaller non-glycosylated and rapidly degraded 57kDa form in ER-Golgi complex. And 70kDa mature form with major catalytic activity located in lysosomes, which is further processed into 52kDa form (PMID:25853898, PMID:26499107).