SMPD2 Rekombinanter Antikörper

SMPD2 Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240400B5

Kat-Nr. : 83520-3-PBS

Synonyme

N-SMase, Neutral sphingomyelinase, Lyso-platelet-activating factor-phospholipase C, Lyso-PAF-PLC, EC:3.1.4.12

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  WB analysis using 83520-3-RR (same clone as 83520-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83520-3-RR (SMPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83520-3-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83520-3-RR (same clone as 83520-3-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug SMPD2 Recombinant antibody (83520-3-RR, Clone:240400B5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83520-3-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83520-3-RR (same clone as 83520-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SMPD2 Recombinant antibody (83520-3-RR, Clone:240400B5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83520-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00518-1

  Cytometric bead array standard curve of MP00518-1, SMPD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83520-1-PBS. Detection antibody: 83520-3-PBS. Standard: Ag7367. Range: 0.78-100 ng/mL.

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  Affinity and Kinetic Characterization of 83520-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83520-3-RR against Human SMPD2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83520-3-PBS bindet in WB, Cytometric bead array, Indirect ELISA SMPD2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SMPD2 fusion protein Ag7367
• Vollständiger Name: sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase)
• Berechnetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC000038
• Gene symbol: SMPD2
• Gene ID (NCBI): 6610
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.