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SMPD3 Polyklonaler Antikörper

SMPD3 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25613-1-PBS

Synonyme

EC:3.1.4.12, Neutral sphingomyelinase 2, Neutral sphingomyelinase II, nSMase 2, nSMase2

Filter:
  • Western Blot (WB) analysis of various lysates using SMPD3 Polyclonal antibody (25613-1-AP)

    WB analysis using 25613-1-AP (same clone as 25613-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25613-1-AP (SMPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25613-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SMPD3 Polyclonal antibody (25613-1-AP)

    WB analysis using 25613-1-AP (same clone as 25613-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 25613-1-AP (SMPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25613-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

25613-1-PBS bindet in WB, Indirect ELISA SMPD3 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SMPD3 fusion protein Ag22487
Vollständiger Name sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase II)
Berechnetes Molekulargewicht 655 aa, 71 kDa
Beobachtetes Molekulargewicht70-71 kDa
GenBank-ZugangsnummerBC112238
Gene symbol SMPD3
Gene ID (NCBI) 55512
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMPD3 (sphingomyelin phosphodiesterase 3), also called neutral sphingomyelinase-2 (nSMase2), is a Golgi-enriched, membrane-bound Zn²⁺-dependent hydrolase that converts sphingomyelin into ceramide and phosphocholine. Ceramide functions as a bioactive lipid second messenger that integrates cellular stress, apoptosis, autophagy and differentiation signals.