SMPDL3B Polyklonaler Antikörper

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• KD/KO Validated

SMPDL3B Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 16552-1-AP

Synonyme

ASM like phosphodiesterase 3b, ASML3B, SMPDL3B

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Galerie der Validierungsdaten

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  WB analysis using 16552-1-AP

  COLO 320 cells lysates were subjected to SDS PAGE followed by western blot with 16552-1-AP (SMPDL3B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16552-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16552-1-AP (SMPDL3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16552-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16552-1-AP (SMPDL3B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16552-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16552-1-AP (SMPDL3B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16552-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16552-1-AP (SMPDL3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16552-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16552-1-AP (SMPDL3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

16552-1-AP bindet in WB, IHC, IF, ELISA SMPDL3B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMPDL3B fusion protein Ag9535
• Vollständiger Name: sphingomyelin phosphodiesterase, acid-like 3B
• Berechnetes Molekulargewicht: 373aa,42 kDa; 455aa,51 kDa
• Beobachtetes Molekulargewicht: 42 kDa, 50-65 kDa
• GenBank-Zugangsnummer: BC014444
• Gene symbol: SMPDL3B
• Gene ID (NCBI): 27293
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMPDL3B is lipid-modulating phosphodiesterase. Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. SMPDL3B acts as a negative regulator of Toll-like receptor signaling.It lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP in vitro (PMID: 26095358). SMPDL3B can be detected 60-70 kDa by N-glycosylation modification (PMID: 26095358, PMID: 31217420).

Publikationen

Species	Application	Title
human	WB	Front Mol Biosci; SMPDL3B Predicts Poor Prognosis and Contributes to Development of Acute Myeloid Leukemia; Authors - Huiqing Qu ; KO Validated
rat	WB,IHC	Basic Clin Pharmacol Toxicol; PMID: 29923306; Authors - Xin Li
mouse	WB	JHEP Rep; Macrophage-derived thrombospondin 1 promotes obesity-associated non-alcoholic fatty liver disease.; Authors - Taesik Gwag
human,pig,monkey	WB,IF	iScience; Porcine reproductive and respiratory syndrome virus upregulates SMPDL3B to promote viral replication by modulating lipid metabolism; Authors - Huan-Huan Shen ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kamal (Verified Customer) (02-15-2024)

This antibody works well for mouse liver tissues.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: Mouse liver tissue