SMS Monoklonaler Antikörper

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SMS Monoklonal Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

1G8E9

Kat-Nr. : 68040-1-PBS

Synonyme

Spermine synthase, 1G8E9, EC:2.5.1.22, MRSR, SPMSY

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  WB analysis using 68040-1-Ig (same clone as 68040-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 68040-1-Ig (SMS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68040-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 68040-1-Ig (same clone as 68040-1-PBS)

  WB result of SMS antibody (68040-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMS transfected K-562 cells. This data was developed using the same antibody clone with 68040-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68040-1-Ig (same clone as 68040-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SMS antibody (68040-1-Ig, Clone: 1G8E9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68040-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68040-1-Ig (same clone as 68040-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SMS (68040-1-Ig, Clone:1G8E9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68040-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68040-1-PBS bindet in WB, IF/ICC, FC (Intra), ELISA SMS und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SMS fusion protein Ag8840
• Vollständiger Name: spermine synthase
• Berechnetes Molekulargewicht: 366 aa, 41 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC009898
• Gene symbol: SMS
• Gene ID (NCBI): 6611
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.