SMUG1 Rekombinanter Antikörper

SMUG1 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240817E4

Kat-Nr. : 83771-5-PBS

Synonyme

UNG3, Single-strand selective monofunctional uracil DNA glycosylase, HMUDG, FDG, EC:3.2.2.-

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  WB analysis using 83771-5-RR (same clone as 83771-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83771-5-RR (SMUG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83771-5-PBS in a different storage buffer formulation.

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  FC experiment of U-2 OS using 83771-5-RR (same clone as 83771-5-PBS)

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug SMUG1 Recombinant antibody (83771-5-RR, Clone:240817E4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83771-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83771-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83771-5-RR against Human SMUG1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83771-5-PBS bindet in WB, FC (Intra), ELISA SMUG1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SMUG1 fusion protein Ag13934
• Vollständiger Name: single-strand-selective monofunctional uracil-DNA glycosylase 1
• Berechnetes Molekulargewicht: 177 aa, 20 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC000417
• Gene symbol: SMUG1
• Gene ID (NCBI): 23583
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UNG(Uracil-DNA glycosylase) removes uracil in DNA resulting from deamination of cytosine or replicative incorporation of dUMP instead of dTMP. Thus, UNG plays a role in suppressing GC-to-AT transition mutations.The UNG gene encodes 2 isoforms that are individually targeted to the mitochondria and the nucleus(PMID:12369930).Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 (HIGM5).