SMURF1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SMURF1 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

Kat-Nr. : 55175-1-AP

Synonyme

E3 ubiquitin-protein ligase SMURF1, EC:2.3.2.26, HECT-type E3 ubiquitin transferase SMURF1, hSMURF1, KIAA1625

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  WB analysis using 55175-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55175-1-AP (SMURF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 55175-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55175-1-AP (SMURF1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 55175-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55175-1-AP (SMURF1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse kidney using 55175-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using SMURF1 antibody (55175-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using 55175-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using SMURF1 antibody (55175-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausnierengewebe, Mausmilzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 4 publications below

Produktinformation

55175-1-AP bindet in WB, IHC, IF-P, IP, CoIP, ELISA SMURF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: SMAD specific E3 ubiquitin protein ligase 1
• Berechnetes Molekulargewicht: 86 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: NM_020429
• Gene symbol: SMURF1
• Gene ID (NCBI): 57154
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMURF1, also named as KIAA1625, is E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. SMURF1 interacts with receptor-regulated SMADs specific for the BMP pathway, SMAD1 and SMAD5, in order to trigger their ubiquitination and degradation and hence their inactivation. The antibody is specific to SMURF1. SMURF1 can be detected as 90-100 kDa when ubiquitinated (PMID:17576816, PMID:20484049).

Publikationen

Species	Application	Title
human		J Cell Biol; A PINCH-1-Smurf1 signaling axis mediates mechano-regulation of BMPR2 and stem cell differentiation.; Authors - Ling Guo
human		Int J Biol Macromol; Dihydroartemisinin requires NR1D1 mediated Rab7 ubiquitination to regulate hepatic stellate cells lipophagy in liver fibrosis; Authors - Zhengyang Bao
rat	WB	Free Radic Biol Med; CKIP-1 affects the polyubiquitination of Nrf2 and Keap1 via mediating Smurf1 to resist HG-induced renal fibrosis in GMCs and diabetic mice kidneys.; Authors - Wenyan Gong
human	WB,IP,IHC	Clin Transl Med; MiRNA-516a promotes bladder cancer metastasis by inhibiting MMP9 protein degradation via the AKT/FOXO3A/SMURF1 axis.; Authors - Yuanyuan Chang
		Br J Pharmacol; Connexin32 ameliorates renal fibrosis in diabetic mice by promoting K48-linked Nox4 polyubiquitination and degradation via the inhibition of Smurf1 expression.; Authors - Zhiquan Chen
human	WB	J Immunol; MLN4924, a First-in-Class NEDD8-Activating Enzyme Inhibitor, Attenuates IFN-β Production.; Authors - Hui Song ; KD Validated