SMURF2 Polyklonaler Antikörper

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• KD/KO Validated

SMURF2 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18038-1-PBS

Synonyme

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  WB analysis using 18038-1-AP (same clone as 18038-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18038-1-AP (SMURF2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18038-1-AP (same clone as 18038-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18038-1-AP (SMURF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 18038-1-AP (same clone as 18038-1-PBS)

  IP result of anti-SMURF2 (IP:18038-1-AP, 4ug; Detection:18038-1-AP 1:300) with mouse testis tissue lysate 4000 ug. This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 18038-1-AP (same clone as 18038-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human malignant melanoma using 18038-1-AP (same clone as 18038-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 18038-1-AP (same clone as 18038-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 18038-1-AP (same clone as 18038-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 18038-1-AP (same clone as 18038-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 18038-1-AP (same clone as 18038-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18038-1-PBS bindet in WB, IHC, Indirect ELISA SMURF2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMURF2 fusion protein Ag12666
• Vollständiger Name: SMAD specific E3 ubiquitin protein ligase 2
• Berechnetes Molekulargewicht: 748 aa, 86 kDa
• Beobachtetes Molekulargewicht: 86-100 kDa
• GenBank-Zugangsnummer: BC093876
• Gene symbol: SMURF2
• Gene ID (NCBI): 64750
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMURF2 is a HECT domain E3 ubiquitin ligase involved in degradation of SMADs,TGF-beta receptor,and other substrates.It also functions in regulation of neuronal and planar cell polarity, induction of senescence, and tumor suppression(PMID:22231558).SMURF2 associates constitutively with SMAD7 and it is nuclear, but binding to SMAD7 induces export and recruitment to activated TGFBR, where it causes degradation of receptors and of SMAD7 via proteasomal and lysosomal pathways(PMIF:11163210). SMURF2 can be detected 100 kDa by poly-sumoylated (PMID:26679521).