SMYD3 Monoklonaler Antikörper

SMYD3 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B5C10

Kat-Nr. : 66330-1-PBS

Synonyme

SMYD3, ZMYND1, ZNFN3A1

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  WB analysis of A431 using 66330-1-Ig (same clone as 66330-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66330-1-Ig (SMYD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66330-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66330-1-Ig (same clone as 66330-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66330-1-Ig (SMYD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66330-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66330-1-Ig (same clone as 66330-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66330-1-Ig (SMYD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66330-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 66330-1-Ig (same clone as 66330-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66330-1-Ig (SMYD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66330-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66330-1-Ig (same clone as 66330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66330-1-Ig (SMYD3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66330-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66330-1-Ig (same clone as 66330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66330-1-Ig (SMYD3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66330-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66330-1-Ig (same clone as 66330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66330-1-Ig (SMYD3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66330-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66330-1-Ig (same clone as 66330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66330-1-Ig (SMYD3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66330-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66330-1-Ig (same clone as 66330-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using SMYD3 antibody (66330-1-Ig, Clone: 1B5C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red). This data was developed using the same antibody clone with 66330-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66330-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SMYD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SMYD3 fusion protein Ag2624
• Vollständiger Name: SET and MYND domain containing 3
• Berechnetes Molekulargewicht: 428aa,49 kDa; 369aa,42 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC031010
• Gene symbol: SMYD3
• Gene ID (NCBI): 64754
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMYD3,also name as ZMYND1 and ZNFN3A1, belongs to the histone-lysine methyltransferase family.It is a histone methyltransferase that plays an important role in transcriptional regulation in human carcinogenesis. It can specifically methylate histone H3 at lysine 4 and activate the transcription of a set of downstream genes, including several oncogenes (e.g., N-myc, CrkL, Wnt10b, RIZ and hTERT) and genes involved in the control of cell cycle.(PMID: 20957523). It plays an important role in transcriptional activation as a member of an RNA polymerase complex. SMYD3 is frequently overexpressed in different types of cancer cells. It functions as a coactivator of Era and potentiates Era activity in response to ligand. SMYD3 as a new coactivator for ER-mediated transcription, providing a possible link between SMYD3 overexpression and breast cancer. (PMID: 19509295) The common variable number of tandem repeats polymorphism in SMYD3 is a susceptibility factor for some types of human cancer(PMID:16155568). Mainly cytoplasmic when cells are arrested at G0/G1, accumulates in the nucleus at S phase and G2/M(Uniprot).