SMYD4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SMYD4 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 17594-1-AP

Synonyme

ZMYND21, SMYD 4, SET and MYND domain-containing protein 4, EC:2.1.1.-

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  WB analysis of HepG2 using 17594-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17594-1-AP (SMYD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 17594-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 17594-1-AP (SMYD4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 17594-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 17594-1-AP (SMYD4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 17594-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 17594-1-AP (SMYD4 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 17594-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 17594-1-AP (SMYD4 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 17594-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 17594-1-AP (SMYD4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

17594-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA SMYD4 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMYD4 fusion protein Ag11552
• Vollständiger Name: SET and MYND domain containing 4
• Berechnetes Molekulargewicht: 804 aa, 89 kDa
• Beobachtetes Molekulargewicht: 89 kDa
• GenBank-Zugangsnummer: BC035077
• Gene symbol: SMYD4
• Gene ID (NCBI): 114826
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMYD4, also named as SET and MYND domain-containing protein 4, is a 804 amino acid protein, which contains 1 MYND-type zinc finger and 1 SET domain. SMYD4 belongs to the class V-like SAM-binding methyltransferase superfamily. SMYD4, as a potential tumor suppressor, plays a critical role in breast carcinogenesis at least partly through inhibiting the expression of Pdgfr-alpha, and could be a novel target for improving treatment of breast cancer. SMYD4 is a muscle-specific transcriptional modulator involved in development.

Publikationen

Species	Application	Title
zebrafish,human	WB,IF,IHC,CoIP	PLoS Genet; The roles of SMYD4 in epigenetic regulation of cardiac development in zebrafish.; Authors - Deyong Xiao ; KO Validated
human	WB,IHC,IF,IP	Cancer Sci; SMYD4 monomethylates PRMT5 and forms a positive feedback loop to promote hepatocellular carcinoma progression; Authors - Zhenyu Zhou
human	WB,IF	Breast Cancer Res; SMYD4 promotes MYH9 ubiquitination through lysine monomethylation modification to inhibit breast cancer progression; Authors - Jin-Shuo Yang