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SNAI1 Rekombinanter Antikörper

SNAI1 Rekombinant Antikörper für IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240164C2

Kat-Nr. : 81584-4-PBS

Synonyme

240164C2, SLUGH 2, SLUGH2, SNAI 1, SNAIL1

Filter:
  • Sandwich ELISA sample test of MP00343-2

    Sandwich ELISA sample test of MP00343-2

    The mean SNAI1 concentration was determined to be 3,819.6 pg/mL in HCT116 cell extract based on a 3.0 mg/mL extract load.

  • Sandwich ELISA standard curve of MP00343-2

    Sandwich ELISA standard curve of MP00343-2

    Sandwich ELISA standard curve of MP00343-2, Human SNAI1 Recombinant Matched Antibody Pair - PBS only. 81584-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag24248. 81584-3-PBS was HRP conjugated as the detection antibody. Range: 31.25-1000 pg/mL.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SNAI1 Recombinant monoclonal antibody (81584-4-RR)

    IF Staining of HeLa using 81584-4-RR (same clone as 81584-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNAI1 antibody (81584-4-RR, Clone: 240164C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81584-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using SNAI1 Recombinant monoclonal antibody (81584-4-RR)

    FC experiment of A549 using 81584-4-RR (same clone as 81584-4-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug SNAI1 Recombinant antibody (81584-4-RR, Clone:240164C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 81584-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00343-2

    Cytometric bead array standard curve of MP00343-2

    Cytometric bead array standard curve of MP00343-2, SNAI1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 81584-4-PBS. Detection antibody: 81584-3-PBS. Standard: Ag24248. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 81584-4-RR

    Affinity and Kinetic Characterization of 81584-4-RR

    Biolayer interferometry (BLl) kinetic assays of 81584-4-RR against Human SNAI1 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

81584-4-PBS bindet in IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA SNAI1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen SNAI1 fusion protein Ag24248
Vollständiger Name snail homolog 1 (Drosophila)
Berechnetes Molekulargewicht 264 aa, 29 kDa
GenBank-ZugangsnummerBC012910
Gene symbol SNAI1
Gene ID (NCBI) 6615
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.