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SNAP25 Polyklonaler Antikörper

SNAP25 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(25)

Kat-Nr. : 14903-1-AP

Synonyme

SNAP-25, SNAP 25, SNAP, RIC-4, RIC4



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMaushirngewebe, Rattenhirngewebe, SH-SY5Y-Zellen
Erfolgreiche IPMaushirngewebe
Erfolgreiche Detektion in IF/ICCSH-SY5Y-Zellen, PC-12-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:10000-1:100000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

14903-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SNAP25 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SNAP25 fusion protein Ag6695
Vollständiger Name synaptosomal-associated protein, 25kDa
Berechnetes Molekulargewicht 23 kDa
Beobachtetes Molekulargewicht 25-27 kDa
GenBank-ZugangsnummerBC010647
Gene symbol SNAP25
Gene ID (NCBI) 6616
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells. In addition, it has been shown that inhibition of neurosecretion by AX type E can be rescued by a SNAP-25 C-terminal peptide, probably by initiating the formation of a fusion competent SNARE complex.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for SNAP25 antibody 14903-1-APProtokoll herunterladen
IF protocol for SNAP25 antibody 14903-1-APProtokoll herunterladen
IP protocol for SNAP25 antibody 14903-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Mol Neurodegener

Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.

Authors - Qingyang Zhang
mouseWB

Free Radic Biol Med

Bavachin ameliorates neuroinflammation and depressive-like behaviors in streptozotocin-induced diabetic mice through the inhibition of PKCδ

Authors - Zhonghong Zhang
ratWB

Front Cell Infect Microbiol

PMID: 28491822

Authors - Anna Piotrowska
mouseWB

Clin Sci (Lond)

Chronic hepatitis C virus infection impairs insulin secretion by regulation of p38δ MAPK-dependent exocytosis in pancreatic β-cells.

Authors - Jizheng Chen
ratWB,IF

Front Mol Neurosci

MicroRNA-210-5p Contributes to Cognitive Impairment in Early Vascular Dementia Rat Model Through Targeting Snap25.

Authors - Zhenxing Ren
mouseWB,IF

Biomedicines

Neuromodulation Induced by Sitagliptin: A New Strategy for Treating Diabetic Retinopathy.

Authors - Hugo Ramos

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

James (Verified Customer) (09-29-2021)

Antibody worked great and was extremely specific to SNAP-25.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Neurogenin 2 iPSC Neurons Day 21
SNAP25 Antibody Western Blot validation (1:1000 dilution) in Neurogenin 2 iPSC Neurons Day 21 (Cat no:14903-1-AP)
FH

Q (Verified Customer) (03-22-2021)

An excellent SNAP25 Ab with a high titer. Very specific and clean.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: human postmortem brain lysate
SNAP25 Antibody Western Blot validation (1:5000 dilution) in human postmortem brain lysate (Cat no:14903-1-AP)