SNAP25 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SNAP25 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Rezensionen (2)

Kat-Nr. : 14903-1-AP

Synonyme

RIC 4, RIC4, SEC9, SNAP, SNAP 25, SNAP25, SUP, Super protein

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Galerie der Validierungsdaten

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  WB analysis of SH-SY5Y using 14903-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14903-1-AP (SNAP25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14903-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14903-1-AP (SNAP25 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 14903-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14903-1-AP (SNAP25 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14903-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14903-1-AP (SNAP25 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14903-1-AP

  IP Result of anti-SNAP25 (IP:14903-1-AP, 3ug; Detection:14903-1-AP 1:3500) with mouse brain tissue lysate 3600ug.

• 

  IF Staining of SH-SY5Y using 14903-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using SNAP25 antibody (14903-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of PC-12 using 14903-1-AP

  Immunofluorescent analysis of PC-12 cells using 14903-1-AP (SNAP25 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of PC-12 using 14903-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using SNAP25 antibody (14903-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen, PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:10000-1:100000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Produktinformation

14903-1-AP bindet in WB, IP, IHC, IF, ELISA SNAP25 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNAP25 fusion protein Ag6695
• Vollständiger Name: synaptosomal-associated protein, 25kDa
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 25-27 kDa
• GenBank-Zugangsnummer: BC010647
• Gene symbol: SNAP25
• Gene ID (NCBI): 6616
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells. In addition, it has been shown that inhibition of neurosecretion by AX type E can be rescued by a SNAP-25 C-terminal peptide, probably by initiating the formation of a fusion competent SNARE complex.

Publikationen

Species	Application	Title
mouse	WB	Mol Neurodegener; Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.; Authors - Qingyang Zhang
rat	WB	Front Cell Infect Microbiol; PMID: 28491822; Authors - Anna Piotrowska
mouse	WB	Clin Sci (Lond); Chronic hepatitis C virus infection impairs insulin secretion by regulation of p38δ MAPK-dependent exocytosis in pancreatic β-cells.; Authors - Jizheng Chen
rat	WB,IF	Front Mol Neurosci; MicroRNA-210-5p Contributes to Cognitive Impairment in Early Vascular Dementia Rat Model Through Targeting Snap25.; Authors - Zhenxing Ren
mouse	WB,IF	Biomedicines; Neuromodulation Induced by Sitagliptin: A New Strategy for Treating Diabetic Retinopathy.; Authors - Hugo Ramos
mouse	WB	Int Immunopharmacol; Chrysin alleviates lipopolysaccharide-induced neuron damage and behavioral deficits in mice through inhibition of Fyn; Authors - Qingchen Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

James (Verified Customer) (09-29-2021)

Antibody worked great and was extremely specific to SNAP-25.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Neurogenin 2 iPSC Neurons Day 21

SNAP25 Antibody Western Blot validation (1:1000 dilution) in Neurogenin 2 iPSC Neurons Day 21 (Cat no:14903-1-AP)

FH

Q (Verified Customer) (03-22-2021)

An excellent SNAP25 Ab with a high titer. Very specific and clean.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: human postmortem brain lysate

SNAP25 Antibody Western Blot validation (1:5000 dilution) in human postmortem brain lysate (Cat no:14903-1-AP)

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