SNAP25 Polyklonaler Antikörper
SNAP25 Polyklonal Antikörper für ELISA
Wirt / Isotyp
Ziege / IgG
Getestete Reaktivität
human, Maus
Anwendung
ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 30003-1-AP
Synonyme
Geprüfte Anwendungen
Produktinformation
30003-1-AP bindet in ELISA SNAP25 und zeigt Reaktivität mit human, Maus
| Getestete Reaktivität | human, Maus |
| Wirt / Isotyp | Ziege / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | Rekombinantes Protein |
| Vollständiger Name | synaptosomal-associated protein, 25kDa |
| Berechnetes Molekulargewicht | 23 kDa |
| GenBank-Zugangsnummer | BC010647 |
| Gene symbol | SNAP25 |
| Gene ID (NCBI) | 6616 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20℃ lagern. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells. In addition, it has been shown that inhibition of neurosecretion by Botox E can be rescued by a SNAP-25 C-terminal peptide, probably by initiating the formation of a fusion competent SNARE complex.
