SNAP25 Rekombinanter Antikörper

SNAP25 Rekombinant Antikörper für IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240069A1

Kat-Nr. : 83259-1-PBS

Synonyme

240069A1, RIC 4, RIC4, RIC-4, SNAP

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  IHC staining of mouse brain using 83259-1-RR (same clone as 83259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83259-1-RR (SNAP25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83259-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83259-1-RR (same clone as 83259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83259-1-RR (SNAP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83259-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-12 using 83259-1-RR (same clone as 83259-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using SNAP25 antibody (83259-1-RR, Clone: 240069A1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83259-1-PBS in a different storage buffer formulation.

• 

  FC experiment of PC-12 using 83259-1-RR (same clone as 83259-1-PBS)

  1x10^6 PC-12 cells were intracellularly stained with 0.25 ug SNAP25 Recombinant antibody (83259-1-RR, Clone:240069A1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83259-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00196-1

  Cytometric bead array standard curve of MP00196-1, SNAP25 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83259-3-PBS. Detection antibody: 83259-1-PBS. Standard: Ag6695. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83259-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 83259-1-PBS against Human SNAP25 was performed. The affinity constant is 2.29 nM.

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  Affinity and Kinetic Characterization of 83259-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83259-1-RR against Human SNAP25 were performed. The affinity constant is 0.322 nM.

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Geprüfte Anwendungen

Produktinformation

83259-1-PBS bindet in IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA SNAP25 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SNAP25 fusion protein Ag6695
• Vollständiger Name: synaptosomal-associated protein, 25kDa
• Berechnetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC010647
• Gene symbol: SNAP25
• Gene ID (NCBI): 6616
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells. In addition, it has been shown that inhibition of neurosecretion by AX type E can be rescued by a SNAP-25 C-terminal peptide, probably by initiating the formation of a fusion competent SNARE complex.