SNAP25 Rekombinanter Antikörper

SNAP25 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240069F12

Kat-Nr. : 83259-5-RR

Synonyme

SNAP 25, SNAP, RIC-4, RIC4, RIC 4

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  WB analysis using 83259-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83259-5-RR (SNAP25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-12 using 83259-5-RR

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using SNAP25 antibody (83259-5-RR, Clone: 240069F12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of SH-SY5Y using 83259-5-RR

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using SNAP25 antibody (83259-5-RR, Clone: 240069F12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83259-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83259-5-RR against Human SNAP25 were performed. The affinity constant is 0.388 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: PC-12-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83259-5-RR bindet in WB, IF/ICC, ELISA SNAP25 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SNAP25 fusion protein Ag6695
• Vollständiger Name: synaptosomal-associated protein, 25kDa
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC010647
• Gene symbol: SNAP25
• Gene ID (NCBI): 6616
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells.