SNAP29 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SNAP29 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(34)

Rezensionen (1)

Kat-Nr. : 12704-1-AP

Synonyme

SNAP-29, CEDNIK, SNAP 29, Synaptosomal-associated protein 29, Vesicle-membrane fusion protein SNAP-29

---

• 

  WB analysis of human kidney using 12704-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12704-1-AP (SNAP29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12704-1-AP

  WB result of SNAP29 antibody (12704-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNAP29 transfected K-562 cells.

• 

  WB analysis of human liver using 12704-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12704-1-AP (SNAP29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12704-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12704-1-AP (SNAP29 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12704-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12704-1-AP (SNAP29 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12704-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12704-1-AP (SNAP29 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 12704-1-AP

  IP result of anti-SNAP29 (IP:12704-1-AP, 4ug; Detection:12704-1-AP 1:600) with Jurkat cells lysate 3200ug.

• 

  IHC staining of mouse kidney using 12704-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12704-1-AP (SNAP29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12704-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12704-1-AP (SNAP29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 12704-1-AP

  Immunohistochemical analysis of paraffin-embedded normal stomach slide using 12704-1-AP (SNAP29 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 12704-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12704-1-AP (SNAP29 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 12704-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12704-1-AP (SNAP29 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach using 12704-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12704-1-AP (SNAP29 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 12704-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12704-1-AP (SNAP29 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of PC-12 using 12704-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using SNAP29 antibody (12704-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 12704-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 12704-1-AP (SNAP29 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 12704-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SNAP29 antibody (12704-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Nierengewebe, HEK-293-Zellen, humanes Lebergewebe, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Nierengewebe, humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: PC-12-Zellen, HEK-293-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 30 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

12704-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA SNAP29 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNAP29 fusion protein Ag3382
• Vollständiger Name: synaptosomal-associated protein, 29kDa
• Berechnetes Molekulargewicht: 258 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC009715
• Gene symbol: SNAP29
• Gene ID (NCBI): 9342
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for the fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. SNAP29 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane. SNAP29 also plays a role in ciliogenesis by regulating membrane fusions.

Publikationen

Species	Application	Title
mouse	WB	Nat Cell Biol; Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.; Authors - Quanlong Lu ; KD Validated
human	WB	Nat Cell Biol; Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.; Authors - Quanlong Lu ; KD Validated
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human	WB	Nat Commun; Kansl1 haploinsufficiency impairs autophagosome-lysosome fusion and links autophagic dysfunction with Koolen-de Vries syndrome in mice.; Authors - Ting Li
human	WB,IF	Nat Commun; KRAS regulation by small non-coding RNAs and SNARE proteins.; Authors - Yonglu Che
human		Autophagy; The ORF7a protein of SARS-CoV-2 initiates autophagy and limits autophagosome-lysosome fusion via degradation of SNAP29 to promote virus replication.; Authors - Peili Hou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Simone (Verified Customer) (03-02-2023)

I used the antibody one time for a westernblot analysis of cells (Stable HeLa cell line expressing sec61b-GFP) which I transfected with siRNA targeting SNAP29 on the one hand and scrambled siRNA on the other hand. I observed a probably specific signal at around 30 kDa, indicated by a strong reduction in the sample from the cells in which I down regulated the protein. I observed a strong unspecific signal at around 40 kDa and weaker unspecific signals at around 70 kDa. I also used the antibody for immunofluorescence one time. I transfected the cells as for the western blot and fixed them with PLP on coverslips and incubated the coverslips overnight with the antibody at 4°C. On the next day I stained the coverslips using an anti rabbit antibody, coupled with Alexa 568 fluorophore. I imaged mainly mitotic cells (see picture attached). I observed a broad staining of the hole cells, leaving out the chromosomal area. I also imaged a few interphase cells, but also observed a rather broad signal. In some interphase cells, especially at the edge of some kind of vesicles I observed a stronger, probably specific staining.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000 for Westernblot and 1:500 for Immunofluorescence
• Cell Tissue Type: Stable HeLa cell line expressing sec61b-GFP

SNAP29 Antibody Western Blot,Immunofluorescence validation (1:2000 for Westernblot and 1:500 for Immunofluorescence dilution) in Stable HeLa cell line expressing sec61b-GFP (Cat no:12704-1-AP)