SNAPIN Rekombinanter Antikörper

SNAPIN Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250300F4

Kat-Nr. : 85949-1-RR

Synonyme

Biogenesis of lysosome-related organelles complex 1 subunit 7, BLOC-1 subunit 7, BLOC1S7, SNAP associated protein, SNAP25BP

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  WB analysis using 85949-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85949-1-RR (SNAPIN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85949-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85949-1-RR against Human SNAPIN were performed. The affinity constant is 7.37 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A375-Zellen, DU 145-Zellen, SKOV-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85949-1-RR bindet in WB, ELISA SNAPIN und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SNAPIN fusion protein Ag0101
• Vollständiger Name: SNAP-associated protein
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 15-18 kDa
• GenBank-Zugangsnummer: BC000761
• Gene symbol: SNAPIN
• Gene ID (NCBI): 23557
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Snapin, a protein of relative molecular weight of 15kd, is implicated in neurotransmission by binding to SNAP-25. Snapin was enriched in neurons and exclusively located on synaptic vesicle membrane, which may be a PKA target for modulating transmitter release through the cAMP-dependent signal-transduction pathway. This anitbody can recognize the 15-18kd(Monomer) and 30-36kd(Dimer) forms of SNAPIN.