SND1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

SND1 Monoklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

CloneNo.

1A6A4

Kat-Nr. : 60265-1-Ig

Synonyme

100 kDa coactivator, EBNA2 coactivator p100, p100, p100 co activator, SND1, TDRD11

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 60265-1-Ig

  WB result of SND1 antibody (60265-1-Ig, 1:60,000) with si-Control and si-SND1 transfected Jurkat cells.

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  WB analysis using 60265-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60265-1-Ig (SND1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60265-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60265-1-Ig (SND1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 60265-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60265-1-Ig (SND1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 60265-1-Ig

  IP Result of anti-SND1 (IP:60265-1-Ig, 5ug; Detection:60265-1-Ig 1:500) with HeLa cells lysate 1400ug.

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  IHC staining of human colon cancer using 60265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60265-1-Ig (SND1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 60265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60265-1-Ig (SND1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 60265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60265-1-Ig (SND1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast hyperplasia using 60265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60265-1-Ig (SND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast hyperplasia using 60265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60265-1-Ig (SND1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60265-1-Ig (SND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60265-1-Ig (SND1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 60265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60265-1-Ig (SND1 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 60265-1-Ig

  Immunofluorescent analysis of HepG2 cells using 60265-1-Ig(SND1 antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-mouse IgG (red).

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  IF Staining of HepG2 using 60265-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SND1 antibody (60265-1-Ig, Clone: 1A6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A431-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, LNCaP-Zellen, NIH/3T3-Zellen, U2OS-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Kolonkarzinomgewebe, humanes Mammahyperplasie-Gewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 5 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

60265-1-Ig bindet in WB, IP, IHC, IF, CoIP, ELISA SND1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SND1 fusion protein Ag1200
• Vollständiger Name: staphylococcal nuclease and tudor domain containing 1
• Berechnetes Molekulargewicht: 101 kDa
• Beobachtetes Molekulargewicht: 101 kDa
• GenBank-Zugangsnummer: BC017180
• Gene symbol: SND1
• Gene ID (NCBI): 27044
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Staphylococcal nuclease domain-containing 1 (SND1), is a multifunctional nuclease that consists of four staphylococcal nuclease domains and a tudor domain. SND1 acts as a coactivator that facilitates transcriptional activity of STAT5, 6 and c-Myc. SND1 is a comprising part of the RNA-induced silencing complex(RISC), and takes part in the functions of miRNA, regulates transcription through transcriptional coactivation, RNA interference, RNA splicing, and RNA editing. Higher level of SND1 has been found in colon cancer and prostate cancer, can promote HCC angiogenesis in xenograft model through induction of angiogenic factors.

Publikationen

Species	Application	Title
human	WB	Cell; SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9; Authors - Nora Schmidt
human	IF	Sci Adv; Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.; Authors - Yuan Wang
human	IF, ELISA	Sci Adv; Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.; Authors - Yuan Wang
human	WB	Elife; The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus.; Authors - Belinda Baquero-Perez ; KD Validated
	IF	J Cell Physiol; The phosphorylation of Tudor-SN mediated by JNK is involved in the regulation of milk protein synthesis induced by prolactin in BMECs.; Authors - Sen Zhang
human	WB,IF,CoIP	J Exp Clin Cancer Res; Circ_0004087 interaction with SND1 promotes docetaxel resistance in prostate cancer by boosting the mitosis error correction mechanism.; Authors - Liang Chen