SNF2L Polyklonaler Antikörper

SNF2L Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29461-1-PBS

Synonyme

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  WB analysis using 29461-1-AP (same clone as 29461-1-PBS)

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 29461-1-AP (SNF2L antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29461-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 29461-1-AP (same clone as 29461-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 29461-1-AP (SNF2L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29461-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-251 using 29461-1-AP (same clone as 29461-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 29461-1-AP (SNF2L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29461-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat lung using 29461-1-AP (same clone as 29461-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 29461-1-AP (SNF2L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29461-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 29461-1-AP (same clone as 29461-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29461-1-AP (SNF2L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29461-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29461-1-PBS bindet in WB, IHC, Indirect ELISA SNF2L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNF2L fusion protein Ag30657
• Vollständiger Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1
• Berechnetes Molekulargewicht: 123 kDa
• Beobachtetes Molekulargewicht: 120-130 KDa
• GenBank-Zugangsnummer: NM_003069
• Gene symbol: SMARCA1/SNF2L
• Gene ID (NCBI): 6594
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SNF2L, also named as SMARCA1, NURF140 and ISWI, encodes the SWI/SNF family of proteins. The encoded protein is an ATPase which is expressed in diverse tissues and contributes to the chromatin remodeling complex that is involved in transcription (PMID: 28801535). SNF2L was shown to promote neurite outgrowth as well as regulate the human engrailed homeotic genes, important regulators of brain development (PMID: 15310751). The protein may also play a role in DNA damage, growth inhibition and apoptosis of cancer cells (PMID: 25462860). SNF2L has 2 isoforms with MW of 123 kDa and 121 kDa, and can be detected as 120-130 kDa.