SNF8 Monoklonaler Antikörper

SNF8 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2C10F2

Kat-Nr. : 67696-1-PBS

Synonyme

2C10F2, Dot3, EAP30, ELL-associated protein of 30 kDa, ESCRT-II complex subunit VPS22

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  WB analysis using 67696-1-Ig (same clone as 67696-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67696-1-Ig (SNF8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 67696-1-Ig (same clone as 67696-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67696-1-Ig (SNF8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67696-1-Ig (same clone as 67696-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67696-1-Ig (SNF8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67696-1-Ig (same clone as 67696-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67696-1-Ig (SNF8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67696-1-Ig (same clone as 67696-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67696-1-Ig (SNF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67696-1-Ig (same clone as 67696-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNF8 antibody (67696-1-Ig, Clone: 2C10F2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67696-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SNF8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SNF8 fusion protein Ag30384
• Vollständiger Name: SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 258 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC008976
• Gene symbol: SNF8
• Gene ID (NCBI): 11267
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.