SNIP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SNIP1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 14950-1-AP

Synonyme

FHA domain-containing protein SNIP1, Smad nuclear-interacting protein 1, SNIP 1

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  WB analysis using 14950-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14950-1-AP (SNIP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14950-1-AP

  IP result of anti-SNIP1 (IP:14950-1-AP, 4ug; Detection:14950-1-AP 1:1000) with HeLa cells lysate 2400ug.

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  IF Staining of HeLa using 14950-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNIP1 antibody (14950-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

14950-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA SNIP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNIP1 fusion protein Ag6772
• Vollständiger Name: Smad nuclear interacting protein 1
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46-50 kDa
• GenBank-Zugangsnummer: BC027040
• Gene symbol: SNIP1
• Gene ID (NCBI): 79753
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Smad nuclear-interacting protein 1 (SNIP1) is a novel interacting factor of the Smad protein family that is expressed in different cell types and is involved in cell proliferation, apoptosis, and differentiation.

Publikationen

Species	Application	Title
human	WB,IHC,IP	Nat Commun; KMT5A-methylated SNIP1 promotes triple-negative breast cancer metastasis by activating YAP signaling.; Authors - Bo Yu ; KO Validated
human	WB	PeerJ; miR-29a-3p directly targets Smad nuclear interacting protein 1 and inhibits the migration and proliferation of cervical cancer HeLa cells.; Authors - Ying Chen ; KD Validated
human	WB,CoIP	bioRxiv; mRNA interactions with disordered regions control protein activity; Authors - Yang Luo
mouse	WB,IP	Nat Commun; SNIP1 and PRC2 coordinate cell fates of neural progenitors during brain development; Authors - Yurika Matsui
human	WB	Cell Death Differ; LncRNA BCAN-AS1 stabilizes c-Myc via N6-methyladenosine-mediated binding with SNIP1 to promote pancreatic cancer; Authors - Guandi Wu
human	WB	J Proteome Res; Quantitative Proteomics Reveal the Mechanism of MiR-138-5p Suppressing Cervical Cancer via Targeting ZNF385A; Authors - Qihang Peng