SNIP1 Rekombinanter Antikörper

SNIP1 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230287D11

Kat-Nr. : 82962-1-PBS

Synonyme

dJ423B22.2, FLJ12553, RP3 423B22.3, SNIP1

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  WB analysis using 82962-1-RR (same clone as 82962-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82962-1-RR (SNIP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82962-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82962-1-RR (same clone as 82962-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNIP1 antibody (82962-1-RR, Clone: 230287D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82962-1-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 82962-1-RR (same clone as 82962-1-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug SNIP1 Recombinant antibody (82962-1-RR, Clone:230287D11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82962-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

82962-1-PBS bindet in WB, IF/ICC, FC (Intra), Indirect ELISA SNIP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SNIP1 fusion protein Ag33543
• Vollständiger Name: Smad nuclear interacting protein 1
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46-50 kDa
• GenBank-Zugangsnummer: BC027040
• Gene symbol: SNIP1
• Gene ID (NCBI): 79753
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Smad nuclear-interacting protein 1 (SNIP1) is a novel interacting factor of the Smad protein family that is expressed in different cell types and is involved in cell proliferation, apoptosis, and differentiation.