Syntaphilin Polyklonaler Antikörper

Syntaphilin Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 13646-1-AP

Synonyme

KIAA0374, SNPH, syntaphilin

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Galerie der Validierungsdaten

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  WB analysis using 13646-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13646-1-AP (Syntaphilin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse trachea using 13646-1-AP

  mouse trachea tissue were subjected to SDS PAGE followed by western blot with 13646-1-AP (Syntaphilin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 13646-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13646-1-AP (Syntaphilin antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13646-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13646-1-AP (Syntaphilin antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Tracheagewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

13646-1-AP bindet in WB, IHC, IF, ELISA Syntaphilin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Syntaphilin fusion protein Ag4594
• Vollständiger Name: syntaphilin
• Berechnetes Molekulargewicht: 538 aa, 58 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa, 65 kDa
• GenBank-Zugangsnummer: BC035788
• Gene symbol: SNPH
• Gene ID (NCBI): 9751
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Syntaphilin is a protein selectively expressed in brain. Syntaphilin competes with SNAP-25 for binding to syntaxin-1 and inhibits SNARE complex formation by absorbing free syntaxin-1, and thereby regulates synaptic vesicle exocytosis (PMID: 10707983). The deduced 537-amino acid syntaphilin protein contains an N-terminal proline-rich domain, a predicted coiled-coil domain, a putative C-terminal transmembrane domain, and numerous consensus sites for protein phosphorylation. Whereas the calculated molecular mass of syntaphilin is 58 kDa, the apparent molecular mass of 70-75 kDa is larger than the predicted mass, suggesting a posttranslational modification of syntaphilin (PMID: 9205841; 10707983). An extra band of 65 kDa was also detected by this antibody that may represent an alternative syntaphilin gene product or a stable degradation product (PMID: 10707983; 24457644).

Publikationen

Species	Application	Title
rat	IF	Sci Rep; Identification of a novel interaction of FUS and syntaphilin may explain synaptic and mitochondrial abnormalities caused by ALS mutations.; Authors - Shaakir Salam
mouse	WB	Mol Brain; Disrupted-in-schizophrenia 1 (DISC1) and Syntaphilin collaborate to modulate axonal mitochondrial anchoring.; Authors - Cana Park
rat	WB	Brain Res; Miro1 provides neuroprotection via the mitochondrial trafficking pathway in a rat model of traumatic brain injury.; Authors - Chen Chen
rat	WB	Bioeng Transl Med; Photobiomodulation augments the effects of mitochondrial transplantation in the treatment of spinal cord injury in rats by facilitating mitochondrial transfer to neurons via Connexin 36; Authors - Zhijie Zhu