SNRPA1 Rekombinanter Antikörper

SNRPA1 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

243097D9

Kat-Nr. : 85649-2-RR

Synonyme

U2 small nuclear ribonucleoprotein A', U2 snRNP A, U2 snRNP A'

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  WB analysis using 85649-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85649-2-RR (SNRPA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 85649-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SNRPA1 antibody (85649-2-RR, Clone: 243097D9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HeLa using 85649-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNRPA1 antibody (85649-2-RR, Clone: 243097D9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen, Mauslebergewebe, Raji-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85649-2-RR bindet in WB, IF/ICC, ELISA SNRPA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SNRPA1 fusion protein Ag10790
• Vollständiger Name: small nuclear ribonucleoprotein polypeptide A'
• Berechnetes Molekulargewicht: 255 aa, 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC022816
• Gene symbol: SNRPA1
• Gene ID (NCBI): 6627
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Small nuclear ribonucleoprotein polypeptide a-prime(SNRPA1) is part of the U2 snRNP particle, which associated with the branch point of the lariat-shaped intron, in intermediate in the cascade of mRNA precursor splicing event. It may be required for cell division, and helps the A' protein to bind stem loop IV of U2 snRNA. This is a rabbit polyclonal antibody raised against the full length SNRPA1 of human origin.