SNRPB Polyklonaler Antikörper

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• KD/KO Validated

SNRPB Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 16807-1-AP

Synonyme

Sm protein B/B, Sm protein B/B', Small nuclear ribonucleoprotein associated proteins B and B', Small nuclear ribonucleoprotein-associated proteins B and B', SmB/B

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  WB analysis using 16807-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16807-1-AP (SNRPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16807-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16807-1-AP (SNRPB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 16807-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16807-1-AP (SNRPB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 16807-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16807-1-AP (SNRPB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16807-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16807-1-AP (SNRPB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16807-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16807-1-AP (SNRPB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16807-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16807-1-AP (SNRPB Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 16807-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16807-1-AP (SNRPB Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 4 publications below
• IP: See 2 publications below

Produktinformation

16807-1-AP bindet in WB, IHC, IP, ELISA SNRPB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNRPB fusion protein Ag10298
• Vollständiger Name: small nuclear ribonucleoprotein polypeptides B and B1
• Berechnetes Molekulargewicht: 234aa,25 kDa; 285aa,30 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC003530
• Gene symbol: SNRPB
• Gene ID (NCBI): 6628
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SNRPB (Small Nuclear Ribonucleoprotein Polypeptides B and B1) is a core component of the spliceosome, a complex molecular machine that plays a critical role in pre-mRNA splicing. This process is essential for the removal of introns and the joining of exons to produce mature mRNA molecules.

Publikationen

Species	Application	Title
human	WB,IP	Elife; Type I and II PRMTs inversely regulate post-transcriptional intron detention through Sm and CHTOP methylation.; Authors - Maxim I Maron
human	IHC	Cell Death Dis; SNRPB promotes the tumorigenic potential of NSCLC in part by regulating RAB26.; Authors - Nianli Liu ; KO Validated
human	IHC	Ann Med; SNRPB and CEP290, predicting the prognosis of diffuse large B cell lymphoma and associated with tumour immune microenvironment; Authors - Jing Tang
human	WB,IHC	Oncogene; The splicing factor SNRPB promotes ovarian cancer progression through regulating aberrant exon skipping of POLA1 and BRCA2; Authors - Yingwei Li ; KD Validated
mouse,human	WB,IHC	Aging (Albany NY); SNRPB promotes the progression of hepatocellular carcinoma via regulating cell cycle, oxidative stress, and ferroptosis; Authors - Xiaoyan Wang
mouse	IP	Adv Sci (Weinh); PRMT5 Maintains Homeostasis of the Intestinal Epithelium by Modulating Cell Proliferation and Survival; Authors - Leilei Li