SNRPB Rekombinanter Antikörper

SNRPB Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250577G8

Kat-Nr. : 86099-1-RR

Synonyme

Sm protein B/B, Sm protein B/B', Small nuclear ribonucleoprotein associated proteins B and B', Small nuclear ribonucleoprotein-associated proteins B and B', SmB/B

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  WB analysis using 86099-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86099-1-RR (SNRPB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 86099-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SNRPB antibody (86099-1-RR, Clone: 250577G8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of K-562 using 86099-1-RR

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using SNRPB antibody (86099-1-RR, Clone: 250577G8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A549-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen, K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86099-1-RR bindet in WB, IF/ICC, ELISA SNRPB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SNRPB fusion protein Ag10298
• Vollständiger Name: small nuclear ribonucleoprotein polypeptides B and B1
• Berechnetes Molekulargewicht: 234aa,25 kDa; 285aa,30 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC003530
• Gene symbol: SNRPB
• Gene ID (NCBI): 6628
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SNRPB (Small Nuclear Ribonucleoprotein Polypeptides B and B1) is a core component of the spliceosome, a complex molecular machine that plays a critical role in pre-mRNA splicing. This process is essential for the removal of introns and the joining of exons to produce mature mRNA molecules.